Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology)
By Unknown Author.
Description
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, cha...
ISBN(s)
1493968858, 9781493968879
